A Brief Review on Swine Flu

 

Aher Vaibhav D*, Arjun Patra, Pronobesh Chattopadhyay and Munesh Mani

College of Pharmacy, IFTM, Moradabad-244001, U.P, India

 

 

ABSTRACT:

Swine influenza is caused by those strains of influenza virus that usually infect pigs and are called swine influenza virus (SIV). Swine influenza is known to be caused by influenza A subtypes H1N1, H1N2, H3N1, H3N2, and H2N3. The main route of transmission is through direct contact between infected and uninfected animals. Airborne transmission through the aerosols produced by pigs coughing or sneezing is also an important means of infection. In pigs influenza infection produces fever, lethargy, sneezing, coughing, difficulty breathing and decreased appetite and in human include fever, cough, sore throat, body aches, headache, chills and fatigue. Risk factors that may contribute to swine-to-human transmission include smoking and not wearing gloves when working with sick animals. The principal method of treatment is vaccination and proper animal management techniques. The modern pork industry also uses antibiotic, which although they have no effect against the influenza virus, do help prevent bacterial pneumonia and other secondary infections in influenza-weakened herds. The U.S. Centers for Disease Control and Prevention recommends the use of Tamiflu (oseltamivir) or Relenza (zanamivir) in human. Vaccines and antivirals will be crucial to the effort, but tracking and communications technologies could also play a key role in monitoring the virus, distributing accurate health information, and quelling outbreaks. Meanwhile, other Internet tools are helping to track the spread of the virus geographically.

 

 

INTRODUCTION:

Swine influenza (also called swine flu, pigfluenza, hog flu, and pig flu) refers to influenza caused by those strains of influenza virus that usually infect pigs and are called swine influenza virus (SIV). Swine influenza is common in pigs in the Midwestern United States (and occasionally in other states), Mexico, Canada, South America, Europe (including the United Kingdom, Sweden, and Italy), Kenya, Mainland China, Taiwan, Japan and other parts of eastern Asia1.

 

The 2009 flu outbreak in humans that is widely known as "swine flu" is due to an apparently virulent new strain of influenza A virus subtype H1N1 that was produced by reassortment from one strain of human influenza virus, one strain of avian influenza virus, and two separate strains of swine influenza. The origin of this new strain is unknown, and the World Organization for Animal Health (OIE) reports that this strain has not been isolated in pigs2. It passes with apparent ease from human to human, an ability attributed to an as-yet unidentified mutation. This 2009 H1N1 strain causes the normal symptoms of influenza, such as fever, coughing and headache3.

 

CLASSIFICATION

Of the three genera of influenza viruses that cause human flu, two also cause influenza in pigs, with Influenzavirus A being common in pigs and Influenzavirus C being rare.4 Influenzavirus B has not been reported in pigs. Within Influenzavirus A and Influenzavirus C, the strains found in pigs and humans are largely distinct, although due to reassortment there have been transfers of genes among strains crossing swine, avian, and human species boundaries4.

 

 


Influenza C:

Influenza C viruses infect both humans and pigs, but do not infect birds5. Transmission between pigs and humans have occurred in the past6. For example, influenza C caused a small outbreaks of a mild form of influenza amongst children in Japan7, and California8. Due to its limited host range and the lack of genetic diversity in influenza C, this form of influenza does not cause pandemics in humans9.

 

Influenza A:

Swine influenza is known to be caused by influenza A subtypes H1N1, H1N210, H3N111, H3N210, and H2N312. In pigs, three influenza A virus subtypes (H1N1, H3N2, and H1N2) are the most common strains worldwide. In the United States, the H1N1 subtype was exclusively prevalent among swine populations before 1998; however, since late August 1998, H3N2 subtypes have been isolated from pigs. As of 2004, H3N2 virus isolates in US swine and turkey stocks were triple reassortants, containing genes from human (HA, NA, and PB1), swine (NS, NP, and M), and avian (PB2 and PA) lineages.13

 

TRANSMISSION:

Transmission between pigs:

The main route of transmission is through direct contact between infected and uninfected animals12. These close contacts are particularly common during animal transport. The direct transfer of the virus probably occurs either by pigs touching noses, or through dried mucus. Airborne transmission through the aerosols produced by pigs coughing or sneezing is also an important means of infection. The virus usually spreads quickly through a herd, infecting all the pigs within just a few days1.

 

Transmission to humans:

People who work with poultry and swine, especially people with intense exposures, are at increased risk of zoonotic infection with influenza virus endemic in these animals, and constitute a population of human hosts in which zoonosis and reassortment can co-occur. Transmission of influenza from swine to humans who work with swine was documented in a small surveillance study performed in 2004 at the University of Iowa15. This study among others forms the basis of a recommendation that people whose jobs involve handling poultry and swine be the focus of increased public health surveillance14

 

SIGNS AND SYMPTOMS:

In swine:

In pigs influenza infection produces fever, lethargy, sneezing, coughing, difficulty breathing and decreased appetite12. In some cases the infection can cause abortion. Although mortality is usually low (around 1-4%) 1, the virus can produce weight loss and poor growth, causing economic loss to farmers. Infected pigs can lose up to 12 pounds of body weight over a 3 to 4 week period.12

 

In humans:

According to the Centers for Disease Control and Prevention (CDC), in humans the symptoms of the 2009 "swine flu" H1N1 virus are similar to those of influenza and of influenza-like illness in general. Symptoms include fever, cough, sore throat, body aches, headache, chills and fatigue. The 2009 outbreak has shown an increased percentage of patients reporting diarrhea and vomiting. The 2009 H1N1 virus is not zoonotic swine flu, as it is not transmitted from pigs to humans, but from person to person15.

 

PREVENTION:

In swine:

Methods of preventing the spread of influenza among swine include facility management, herd management, and vaccination. Because much of the illness and death associated with swine flu involves secondary infection by other pathogens, control strategies that rely on vaccination may be insufficient.

 

Control of swine influenza by vaccination has become more difficult in recent decades, as the evolution of the virus has resulted in inconsistent responses to traditional vaccines. Standard commercial swine flu vaccines are effective in controlling the infection when the virus strains match enough to have significant cross-protection, and custom (autogenous) vaccines made from the specific viruses isolated are created and used in the more difficult cases.16, 17. Present vaccination strategies for SIV control and prevention in swine farms; typically include the use of one of several bivalent SIV vaccines commercially available in the United States. Of the 97 recent H3N2 isolates examined, only 41 isolates had strong serologic cross-reactions with antiserum to three commercial SIV vaccines. Since the protective ability of influenza vaccines depends primarily on the closeness of the match between the vaccine virus and the epidemic virus, the presence of nonreactive H3N2 SIV variants suggests that current commercial vaccines might not effectively protect pigs from infection with a majority of H3N2 viruses18, 19.

 

Prevention of pig to human transmission (Fig. 1)

Swine can be infected by both avian and human influenza strains of influenza, and therefore are hosts where the antigenic shifts can occur that create new influenza strains. The transmission from swine to human is believed to occur mainly in swine farms where farmers are in close contact with live pigs. Although strains of swine influenza are usually not able to infect humans this may occasionally happen, so farmers and veterinarians are encouraged to use a face mask when dealing with infected animals. The use of vaccines on swine to prevent their infection is a major method of limiting swine to human transmission. Risk factors that may contribute to swine-to-human transmission include smoking and not wearing gloves when working with sick animals.20

 

Prevention of human to human transmission (Fig. 1)

Influenza spreads between humans through coughing or sneezing and people touching something with the virus on it and then touching their own nose or mouth.  Swine flu cannot be spread by pork products, since the virus is not transmitted through food21. The swine flu in humans is most contagious during the first five days of the illness although some people, most commonly children, can remain contagious for up to ten days. Diagnosis can be made by sending a specimen, collected during the first five days for analysis. Experts agree that hand-washing can help prevent viral infections, including ordinary influenza and the swine flu virus. Influenza can spread in coughs or sneezes, but an increasing body of evidence shows small droplets containing the virus can linger on tabletops, telephones and other surfaces and be transferred via the fingers to the mouth, nose or eyes. Alcohol-based gel or foam hand sanitizers work well to destroy viruses and bacteria.


 

Figure 1: Prevention of swine flu: Prevention of pig to human transmission and Prevention of human to human transmission

 

 


Anyone with flu-like symptoms such as a sudden fever, cough or muscle aches should stay away from work or public transportation and should contact a doctor to be tested.

 

Social distancing is another tactic. It means staying away from other people who might be infected and can include avoiding large gatherings, spreading out a little at work, or perhaps staying home and lying low if an infection is spreading in a community. Public health and other responsible authorities have action plans which social distancing actions to request or require depending on the severity of the outbreak22.

TREATMENT:

In swine:

As swine influenza is not usually fatal to pigs, little treatment is required. Instead veterinary efforts are focused on preventing the spread of the virus throughout the farm, or to other farms.12 Vaccination and animal management techniques are most important in these efforts. The modern pork industry also uses antibiotic, which although they have no effect against the influenza virus, do help prevent bacterial pneumonia and other secondary infections in influenza-weakened herds23.

 

In humans:

If a person becomes sick with swine flu, antiviral drugs can make the illness milder and make the patient feel better faster. They may also prevent serious flu complications. For treatment, antiviral drugs work best if started soon after getting sick (within 2 days of symptoms). Beside antivirals, palliative care, at home or in the hospitals, focuses on controlling fevers and maintaining fluid balance. The U.S. Centers for Disease Control and Prevention recommends the use of Tamiflu (oseltamivir) or Relenza (zanamivir) for the treatment and/or prevention of infection with swine influenza viruses, however, the majority of people infected with the virus make a full recovery without requiring medical attention or antiviral drugs.24 The virus isolates in the 2009 outbreak have been found resistant to amantadine and rimantadine.25

 

In the U.S., on April 27, 2009, the FDA issued Emergency Use Authorizations to make available Relenza and Tamiflu antiviral drugs to treat the swine influenza virus in cases for which they are currently unapproved26.

Flu vaccine acceptance

 

According to the CDC: Influenza vaccination is the primary method for preventing influenza and its severe complications. Vaccination is associated with reductions in influenza-related respiratory illness and physician visits among all age groups, hospitalization and death among persons at high risk, otitis media among children, and work absenteeism among adults. Although influenza vaccination levels increased substantially during the 1990s, further improvements in vaccine coverage levels are needed.27

 

CURRENT STATUS:

Influenza research includes molecular virology, molecular evolution, pathogenesis, host immune responses, genomics, and epidemiology. These help in developing influenza countermeasures such as vaccines, therapies and diagnostic tools. Improved influenza countermeasures require basic research on how viruses enter cells, replicate, mutate, evolve into new strains and induce an immune response. The Influenza Genome Sequencing Project is creating a library of influenza sequences that will help us understand what makes one strain more lethal than another, what genetic determinants most affect immunogenicity, and how the virus evolves over time. Solutions to limitations in current vaccine methods are being researched28.

 

The Influenza Genome Sequencing Project (IGSP) is an American-based genome project aimed at improving the availability of genomic sequence data from influenza viruses and related information. The project is funded by the National Institute of Allergy and Infectious Diseases (NIAID), a division of the National Institutes of Health (NIH), and has been operating out of the NIAID Microbial Sequencing Center at The Institute for Genomic Research (TIGR). Sequence information generated by the project has been placed into public domain through Gene Bank. The project completed its first genomes in March 2005 and has rapidly accelerated since, with over 1800 genomes now in the public domain. As of January 2007, 1813 isolates had been completely sequenced from influenza viruses that are endemic in human (human flu) avian (bird flu) and swine (swine flu) populations, including many strains of H3N2 (human), H1N1 (human), and H5N1 (avian)29.

Variants of Influenza virus A

 

Variants are sometimes named according to the species (host) the strain is endemic in or adapted to some variants named using this convention are

·                  Bird flu

·                  Human Flu

·                  Swine Flu

·                  Horse Flu

·                  Dog Flu

Avian variants have also sometimes been named according to their deadliness in poultry, especially chickens:

·                  Low Pathogenic Avian Influenza (LPAI)

·                  Highly Pathogenic Avian Influenza (HPAI), also called deadly flu or death flu Influenza A viruses are negative sense, single-stranded, segmented RNA viruses. There are 16 different HA antigens (H1 to H16) and nine different NA antigens (N1 to N9) for influenza A. Until recently, 15 HA types had been recognized, but a new type (H16) was isolated from black-headed gulls caught in Sweden and the Netherlands in 1999 and reported in the literature in 200530.

 

CONCLUSION:
Swine flu is a dreaded disease and its treatment is not certain. So it’s important to gather information on the outbreak, spreading and fighting of the so-called swine flu; and   we have to see the increasing transmission, particularly human-to-human across the world as it's going to be increasingly difficult for us to prevent this disease. This review focuses on what is known about the basic concept of swine flu: its sources, classification, transmission, symptoms, prevention and treatment in swine and human. Also current status of pathogenicity and spread of influenza A virus in species other than man, with particular emphasis on the wild bird reservoir. A drug development programme should be undertaken to develop modern drugs for the treatment of the disease.

 

REFERENCES:

1.       Swine influenza. The Merck Veterinary Manual. 2008. http://www.merckvetmanual.com/mvm/index.jsp .

2.       V Trifonov, H Khiabanian, B Greenbaum, R Rabadan. The origin of the recent swine influenza A(H1N1) virus infecting humans. Eurosurveillance. 2009; 4 (17). http://www.eurosur veillance.org/images/dynamic.

3.       Maria Zampaglione. Press Release: A/H1N1 influenza like human illness in Mexico and the USA: OIE statement. 2009.  World Organisation for Animal Health. http://www.oie. int/eng/press

4.       Heinen PP. Swine influenza: a zoonosis. Veterinary Sciences Tomorrow. Influenza B and C viruses are almost exclusively isolated from man, although influenza C virus has also been isolated from pigs and influenza B has recently been isolated from seals. 2003.

5.       Bouvier NM, Palese P. The biology of influenza viruses. Vaccine. 2008; 26 (4): 49–53.

6.       Kimura H, Abiko C, Peng G. Interspecies transmission of influenza C virus between humans and pigs. Virus Res. 1997.48 (1):71–79. http://linkinghub.elsevier.com/retrieve/pii/

7.       Matsuzaki Y, Sugawara K, Mizuta K. Antigenic and genetic characterization of influenza C viruses which caused two outbreaks in Yamagata City, Japan, in 1996 and 1998. J. Clin. Microbiol. 2002; 40 (2): 422–429. http://jcm.asm.org/cgi/pmidlookup.

8.       Lynch JP, Walsh EE. Influenza: evolving strategies in treatment and prevention. Semin Respir Crit Care Med .2007; 28 (2) 144–158.

9.       Swine Influenza. Swine Diseases (Chest). Iowa State University College of Veterinary Medicine. http:// www.vetmed.iastate.edu/departments/vdpam/swine/diseases/chest/swineinfulenza/

10.     Shin JY, Song MS, Lee EH, Lee YM, Kim SY, Kim HK, Choi JK, Kim CJ, Webby RJ, Choi YK .Isolation and characterization of novel H3N1 swine influenza viruses from pigs with respiratory diseases in Korea. Journal of Clinical Microbiology.2006; 44 (11): 3923–3927.

11.     Ma W, Vincent AL, Gramer MR, Brockwell CB, Lager KM, Janke BH, Gauger PC, Patnayak DP, Webby RJ, Richt JA . Identification of H2N3 influenza A viruses from swine in the United States. Proc Nat Acad Sci USA .2007; 104 (52): 20949–20954.

12.     Kothalawala H, Toussaint MJ, Gruys E. An overview of swine influenza. 2006; Vet Q 28 (2) 46–53.

13.     Yassine HM, Al-Natour MQ, Lee CW, Saif YM. Interspecies and intraspecies transmission of triple reassortant H3N2 influenza A viruses. Virol J.2007. 28 (4): 129.

14.     Gray GC, Kayali G. Facing pandemic influenza threats: the importance of including poultry and swine workers in preparedness plans. Poultry Science. 2009; 88 (4) 880–884.

15.     Swine Flu and You. CDC. http://www.cdc.gov/swineflu/ swineflu you.htm. Swine flu virus turns endemic. National Hog Farmer. http://nationalhogfarmer.com/mag/swine flu virus endemic.

16.     Swine. Custom Vaccines. Novartis. http://www.livestock. novartis.com/cv swine.html.

17.     Gramer Marie Renι, Lee Jee Hoon, Choi Young Ki, Goyal Sagar M, Joo Han Soo. Serologic and genetic characterization of North American H3N2 swine influenza A viruses. Canadian Journal of Veterinary Research. 2007; 71 (3) 201–206. http://www.pubmedcentral.nih.gov/

18.     Myers KP, Olsen CW, Gray GC. Cases of swine influenza in humans: a review of the literature. Clin Infect Dis. 2007; 44 (8) 1084–1088.

19.     Ramirez A, Capuano AW, Wellman DA, Lesher KA, Setterquist SF, Gray GC. Preventing zoonotic influenza virus infection . Emerging Infect. Dis. 2006; 12 (6): 996–1000. http://www.cdc.gov/ncidod/eid.

20.     Q and A: Key facts about swine influenza (swine flu) Spread of Swine Flu. Centers for Disease Control and Prevention. 2009. http://www.cdc.gov/swineflu/key facts.htm.

21.     Q and A: Key facts about swine influenza (swine flu) Diagnosis. Centers for Disease Control and Prevention. 2009. http://www.cdc.gov/swineflu/key facts.htm.

22.     Influenza Factsheet. Center for Food Security and Public Health, Iowa State University.  http://www.cfsph.iastate.edu/ Factsheets/pdfs/influenza.pdf.

23.     www.who.int/csr/disease/swineflu/faq/en/index.html;http://www.who.int/csr/disease/swineflu/faq/en/index.html.

24.     Antiviral Drugs and Swine Influenza. Centers for Disease Control. http://www.cdc.gov/swineflu/antiviral swine.htm.

25.     FDA Authorizes Emergency Use of Influenza Medicines, Diagnostic Test in Response to Swine Flu Outbreak in Humans. FDA News, April 27, 2009. Fda.gov. 2009-04-27. http://www.fda.gov/bbs/topics/NEWS/2009/NEW02002.html.

26.     CDC report Prevention and Control of Influenza published April 12, 2002.

27.     CIDRAP article Record flu vaccine supply expected next season published January 26, details current availability in the US.

28.     http://msc.jcvi.org/influenza/index.shtml

29.     http://www3.niaid.nih.gov/LabsAndResources/resources/mscs/default.htm

30.     http://en.wikipedia.org/wiki/Influenzavirus A

 

Received on 21.09.2009

Accepted on 18.11.2009     

© A&V Publication all right reserved

Research J. Pharmacology and Pharmacodynamics 2(2): March –April 2010: 126-130